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Study on the interaction between a luminescent metal-ligand probe and bovine serum albumin by fluorescence spectroscopy

Author:Swarnalatha Kalaiyar and Godlyn Abraham

Abstract:
The interaction between a luminescent metal-ligand probe, [Ru(dpp)3]2+ (dpp = 4,7- diphenyl-1,10- phenanthroline disulphonic acid) with bovine serum albumin(BSA) was investigated by means of absorption and fluorescence spectroscopy. The fluorescence of BSA was quenched by the ruthenium (Ð†Ð†) complexes and the quenching mechanism was considered as static by forming a complex. The association constant Kb and the number of binding sites, n were calculated using the modified Stern Volmer equation. The fluorescence of the metal complex is highly enhanced by the addition of BSA. In addition, the results of synchronous fluorescence spectra showed that binding of the metal probe with the BSA induce conformational changes in BSA. The fluorescent enhancement of the system is found to be originated from the hydrophobic microenvironment provided by BSA.